克式症候群患者接受顯微取精手術與單一精子卵質顯微注射之子代追蹤
林巧文1、黃志賢123
1臺北榮民總醫院 泌尿部; 2國立陽明大學醫學院泌尿學科;3書田泌尿科學研究中心
Outcome of Intracytoplasmic Sperm Injection with Fresh Sperm Retrieved by mTESE in Klinefelter Syndrome Patients:
Our Experience in Taipei Veteran General Hospital
Chyau-Wen Lin 1, William J. Huang123
1Department of Urology, Taipei Veterans General Hospital;
2Department of Urology, School of Medicine, National Yang-Ming University, Taipei, Taiwan;
3Shu-Tien Urological Institute, National Yang-Ming University, Taipei, Taiwan
Purpose
Klinefelter syndrome (KS), 47 XXY, is one of the most common etiology of male infertility. For those patients with azoospermia, testicular sperm extraction (TESE) or microscopic TESE (mTESE) is now wildly applied as a part of assisted reproductive techniques. However, there are scarce studies regarding to the karyotype of their offspring.
Material and Methods
In retrospective fashion, the patients who are diagnosed with KS and received artificial reproductive technologies at Taipei Veteran General Hospital from 2010-2019. The basic hormone profiles of both mothers and fathers were obtained before the treatment. Diagnostic mTESE was first done as pioneer evaluation of the potential sperm location. Therapeutic mTESE was then performed, and the retrieved testis tissue was processed and sperm was injected into frozen or freshly retrieved oocyte. Embryo transfer was then performed by obstetrician. Prenatal care was followed as Taiwan National Health Insurance guideline, and amniocentesis with further karyotyping was performed as the parents’ wishes.
Results
There were 26 couples who received the mTESE + intracytoplasmic sperm injection (ICSI) treatment in our hospital during 2010-2019. To date, 7 couples reporting live births, with two pairs of twins. Of 9 offspring, 5 didn’t received prenatal karyotyping. Two were 46 XX and two were 46XY. All children reported no abnormality in the postnatal screening.
Conclusion
This study showed that patients with Klinefelter Syndrome could father both phenotypically and genotypically normal children under the assistance of mTESE and ICSI.