人類絨毛膜激素在移植動物模式中對耐受性樹突細胞形成之影響
陳慧雅、陳志碩、林威宇、黃雲慶、林健煇、黃國財、何東儒
嘉義長庚醫院 外科部 泌尿科
Effect of Human Chorionic Gonadotropin on Tolerogenic Dendritic Cell Formation in Transplantation Animal Model
Wai-Nga Chan, Chih-Shou Chen, Wei-Yu Lin, Yun-Ching Huang,
Jian-Hui Lin, Kuo-Tsai Huang, Dong-Ru Ho
Division of Urology, Department of Surgery, Chiayi Chang Gung Memorial Hospital, Taiwan
Purpose: Dendritic cells (DCs) are specialized antigen presenting cells that build up the bridge between innate and adaptive immunity. We hope to reveal the effect of beta human chorionic gonadotropin (b-hCG) on dendritic cell/ T cell interaction, and on autologous tissue transplantation based on mice model.
Materials and Methods: We first check the effect of b-hCG on DCs and its induced maturation. Then we test how b-hCG affects T cell function. Last, we evaluate the combined effect of b-hCG on cellular and serous immune response in transplantation animal model through dendritic cell modification. T cell was retrieved with magnetic beads and its profiles checked with flowcytometry. The effect of b-HCG on cytokine profiles was checked with cytokine array.
Results: We found that mature dendritic cell subcutaneous injection will induce systemic spleen cytokines increase including IL-10, TNF-a, INF-r, IL-18, IL-33, Casp1, and IL-1b; but only TNF-a reached significant level. When combined with b-hCG treatment, TNF-a, IL-10, Casp1, and IL-1b would increase even more prominent, while IFN-r and IL-6 decreased. The percentage of cytotoxic, regulatory, and helper (Th1) T cell would increase when DCs are implanted, but it would decrease when dendritic cells comes from b-hCG pretreated mice. Generally speaking, b-hCG tend to promote Th2 and related cytokine immune response, and decrease Th1 response along with related cytokines.
Conclusions: Our study showed that b-hCG will affect mature dendritic cell conducted by solid organ transplantation, and will resulting in increase of systemic TNF-a and IL-10 concentration, and decrease in cytotoxic T cell ratio