探討Rutin於攝護腺癌的調控與治療之機轉

郭威廷^1,3、麥倖嘉¹、吳振宇^1,4、吳俊賢^1,2,3,4、林嘉祥^1,2,3,4

義大醫院泌尿科¹；義守大學醫學院護理學系²

義守大學化學工程學系暨生物科技與化學工程研究所³

義守大學國際醫學系⁴

Anti-Cancer Effect of Rutin in Human Prostate Cancer Cell

Wade Wei-Ting Kuo^1,3, Hsing-Chia Mai¹, Richard C. Wu^1,4, Chun-Hsien Wu^1,2,3,4, Victor C. Lin^1,2,3,4

Department of Urology, E-DA hospital, Kaohsiung, Taiwan¹

Department of Nursing, I-Shou University, Kaohsiung, Taiwan²

Department of Chemical Engineering and Institute of Biotechnology and Chemical Engineering, I-Shou University, Kaohsiung, Taiwan³

School of Medicine, College of Medicine, I-Shou University, Kaohsiung, Taiwan⁴

Purpose:

Rutin has been demonstrated their antitumor activity by modulating various macromolecular targets. This study will explore the regulation of rutin to the prostate cancer.

Materials and Methods:

The LNCap and LNCap Enz-R (already stimulated with Enzalutamide for more than 6 months) were treated with various concentrations of rutin (0, 25, 50, 100 μM) for 24 hours followed by the examination of cell viability using MTT and trypan blue exclusion assays. Cell migration was assessed by wound healing assay. We investigated the ROS production, mitochondrial biogenesis and mitochondrial marker (e.q. AMPK) during the progression of prostate cancer by kit and Western blotting assay. Western blot and immunofluorescence staining were used to detect the expression of epithelial-mesenchymal transition (EMT)-associated factors, EMT signaling and AMPK axis.

Results:

Rutin treatment significantly inhibited cell proliferation and migration of both LNCap and LNCap-Enz-R cells. In addition, rutin enhanced SOD activity-mediated modifications in both LNCap and LNCap-Enz-R cells. Also, we showed that the expression of mitochondrial biogenesis of prostate cells was upregulated by rutin. We demonstrated that rutin exhibited the anticancer effects on prostate cancer via inhibition of expression of EMT marker, Snail and Slug, with increasing expression of E-cadherin. More importantly, treatment with rutin significantly increased the expression of mitochondrial factor subsequent to induced AMPK activation.

Conclusion:

These findings indicate that rutin can be effectively inhibited prostate cancer cell by means of various macromolecular targets and can be a promising adjunctive therapy for prostate cancer treatment.