水凝膠包覆胜肽誘餌對於老鼠膀胱出口阻塞導致膀胱傷害之療效

沈運筑¹、石宏仁¹、黃俊仁^２

彰化基督教醫院 外科部 泌尿科¹; 萬芳醫院 麻醉部²

The Therapeutic Effects of Peptide Decoy-Loaded Hydrogel in Mice with Bladder Outlet Obstruction-Induced Bladder Injury

Yun-Chu Shen¹, Hung-Jen Shih¹, Chun-Jen Huang²

¹Divisions of Urology, Department of Surgery, Changhua Christian Hospital, Changhua, Taiwan; ²Department of Anesthesiology, Wan Fang Hospital, Taipei, Taiwan

Purpose:

With the increases in aging population, the case number of bladder dysfunction induced by bladder outlet obstruction is significantly increased in recent years. The novel and effective therapy against bladder dysfunction induced by bladder outlet obstruction is desperately needed. KCF18 is a novel peptide decoy therapy with potent anti-inflammatory effects. KCF18 loaded in hydrogel provides a sustained release of functional molecular with therapeutic effects (i.e., KCF18) and this kind of therapy is suitable for chronic disease therapy. This project will be the first study to investigate the therapeutic effects of novel therapy with peptide decoy (KCF18)-loaded hydrogel against bladder injury induced by bladder outlet obstruction.

Materials and Methods:

Adult male BALB/c mice (8 weeks; National Laboratory Animal Center, Taipei, Taiwan) will be used will be used for partial urethral obstruction experiments. Mice will receive sham or BOO operation on the first day of experiment. To evaluate the treatment timing post BOO, three treatment time points (early phase: 7th day after operation; middle phase: 14th day after operation; late phase: 28th day after operation) will be selected for study. Bladder functional assessment will be performed by mice voiding behavior analysis and awake cystometry. The fibrosis-related and proinflammatory markers will be evaluated by immunohistochemistry in bladder tissues.

Results:

KCF18-loaded hydrogels can be sustained release for up to 7 days. The results of immunohistochemistry study demonstrate significant KCF18-biotin complex expression in the bladder submucosa and muscle layers after KCF18-loaded hydrogel therapy. The micturition function and fibrosis were significant improved in early treatment (7 days) with KCF18-loaded hydrogel treatment group, but not in middle and late phase groups.

Conclusion:

KCF18-loaded hydrogel therapy significantly mitigated the BOO-induced bladder injury. However, the therapy should be used in the early stage.