利用次世代定序技術和生物資訊找出綠茶兒茶素影響膀胱癌細胞凋亡的重要基因
李香瑩1,2,3、李威明1,3,4、李經家1,3、柯宏龍1,3、吳文正1,3、郭柏麟1
1高雄醫學大學臨床醫學所
2高雄市立大同醫院 泌尿科
3高雄醫學大學附設中和紀念醫院 泌尿部
4衛生福利部屏東醫院 泌尿科
Deduction of genes potentially involving in urothelial carcinoma apoptosis by EGCG using next-generation sequencing and bioinformatics approaches
Hsiang ying Lee1,2,3, Wei-Ming Li1,3,4, Ching-Chia Li1,3, Hung-Lung Ke1,3, Po-Lin Kuo1
1Graduate Institute of Clinical Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan
2Department of Urology, Kaohsiung Municipal Ta-Tung Hospital, Kaohsiung, Taiwan
3Department of Urology, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan
4 Department of Urology, Ministry of Health and Welfare Pingtung Hospital, Pingtung, Taiwan
Purpose:
Bladder cancer is one of the most common cancers among people, which ranked the 11th incidence cancer. The incidence rate of urothelial carcinoma presents varied difference between countries, that is because the exposure of circumstance factors and pollutions. The higher incidence happened in the black-foot disease endemic area in Taiwan where residents are under chronic arsenic exposure through drinking well water. From the disease process of bladder cancer, we need monitor the possibility of recurrence and progression. Because of high recurrence rate of bladder cancer, even for earlier stage, regular follow-up strategy is important.
Materials and Methods:
Epigallocatechin-3-gallate (EGCG) is the most abundant and bioactive polyphenol in green tea. Many previous studies demonstrated the protection role of EGCG in various cancers through promoting tumor cell apoptosis. Although some research have identified EGCG inhibit bladder cancer cell proliferation, migration and apoptosis, as to my knowledge, evaluation through next-generation sequencing and bioinformatics is the first time. We extracted mRNA, miRNA from BFTC-905 cell line without EGCG and BFTC-905 cell line with EGCG then compare its expression to identify significant difference of candidate gene and upstream regulator by using bioinformatics database.
Results:
Through GEO: GSE32894 platform we detected ARRB1 and DLG2 present higher expression in advanced bladder cancer tissue than non-muscle invasive bladder cancer which indicated palys an improtant role in oncogenesis in urothelial carcinoma. By using MiRmap and Oncomine database, we identified the important role of these candidate genes. BFTC-905 cell line has specific origin from black-foot area in Taiwan which presents unique feature.
Conclusion:
Based on current study to discover the mechanism of EGCG in bladder cancer, it will become a representative study in Taiwan. Therefore, this study will identify novel target of prevention and treatment for urothelial carcinoma.