輔酶Q10對慢性腎臟病模式之雄鼠生殖功能之影響
曹智惟1 許育瑞2 吳勝堂1 查岱龍1 劉沁瑜3
1國防醫學院 三軍總醫院 外科部 泌尿外科2國防醫學院 三軍總醫院 內科部 腎臟內科3輔仁大學 營養科學系
The role of coenzyme Q10 on the reproductive function of male mice model of chronic kidney disease
Chih-Wei Tsao1, Yu-Juei Hsu2, Sheng-Tang Wu1, Tai-Lung Cha1 and Chin-Yu Liu3
1Division of Urology, Department of Surgery, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan
2 Division of Nephrology, Department of Medicine, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan
3 Department of Nutritional Science, Fu Jen Catholic University, New Taipei, Taiwan
Purpose:
We aimed to explore the effect of coenzyme Q10 on testicular oxidative stress and reproductive function in the chronic kidney disease.
Materials and Methods:
Nine-week-old C57BL/6 male mice were randomly divided into five groups: normal control group (NC group), sham-operated group received corn oil (SC group), sham-operated group received coenzyme Q10 (SQ group), the CKD-operated group received corn oil (CC group) and the CKD-operated group received coenzyme Q10 (CQ group). Chronic kidney disease was induced by 5/6 nephrectomy and the coenzyme Q10 was dissolved in corn oil fed by gavage. After eight weeks feeding, mice were sacrificed. Results:
The concentration of coenzyme Q10 both in serum and testis were decreased in the CC group, and increased in the CQ group. In testosterone biosynthesis pathway, steroidogenic acute regulatory (StAR) protein expression was decreased in the CC group and increased in the CQ group. The immaturity of testicular cells and poor sperm quality of CKD mice also improved after eight-week coenzyme Q10 feeding. In addition, glutathione peroxidase (GPx) and catalase (CAT) activity could increase in the CQ group.
Conclusion:
CKD mice with coenzyme Q10 supplementation have increased StAR protein expression in testosterone biosynthesis pathway, antioxidant activity, better sperm quality and the testicular cells morphology than CKD mice without coenzyme Q10 feeding.