生酮飲食對高脂高膽固醇飲食誘導之雄性小鼠生殖功能的影響
曹智惟1 張庭嘉2 林士祥3,4 吳勝堂1 查岱龍1 劉沁瑜2
1國防醫學院 三軍總醫院 外科部 泌尿外科2輔仁大學 營養科學系3台北醫學大學 保健營養學系4台北醫學大學 食品安全碩士學位學程
Effects of ketogenic diet on the reproductive function in high fat and high cholesterol diet-induced male mice
Chih-Wei Tsao1, Ting-Chia Chang2, Shyh-Hsiang Lin3,4, Sheng-Tang Wu1, Tai-Lung Cha1 and Chin-Yu Liu2
1Division of Urology, Department of Surgery, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan
2Department of Nutritional Science, Fu Jen Catholic University, New Taipei, Taiwan
3School of Nutrition and Health Sciences, College of Nutrition, Taipei Medical University, Taipei, Taiwan
4Master of Program of Food Safety, College of Nutrition, Taipei Medical University, Taipei, Taiwan
Purpose:
Medium-chain triacylglycerol (MCT) oil, a common source of dietary fat in ketogenic diet, can significantly decrease body fat, lipid levels and glucose due to a different metabolism pathway compared with long-chain triacylglycerol (LCT). To investigate the effects of ketogenic diet on metabolic dysfunction, testicular antioxidant capacity, apoptosis, inflammation and spermatogenesis in high-fat and high cholesterol dieting male mice.
Materials and Methods:
Male C57BL/6 mice (n=42) were fed with two different diets: normal diet (NC group, n=10) or high fat diet plus cholesterol (HFC, n=32) diet. After 16 weeks of high fat diet to establish a diet-induced obesity mice model, obese mice then divided into two groups, one was kept the original diet (HFC group, n=16); the other fed with the MCT-based ketogenic diet for 8 weeks (KD group, n=16).
Results:
Compared to the HFC group, mice changed into ketogenic diet increased ketone bodies and improved the metabolic profile and fatty liver. Also, HFC-induced decreases in motility and normal morphology of sperm and worse maturity of testicular cells were improved by the ketogenic diet. Both the HFC and KD group had higher estradiol and lower 17β-HSD protein expression. In addition, obese mice fed with ketogenic diet reduced lipid peroxidation and increased glutathione peroxidase. However, the HFC and KD group increase protein expression of apoptosis and inflammation in the testis.
Conclusion:
Different dietary patterns of high fat diet would induce different levels of oxidative stress, cause apoptosis and inflammation, and thus damage the testicular function and semen quality.