以小鼠單側輸尿管阻塞模式評估小分子褐藻糖膠預防腎臟纖維化的效果

王宗偉^1,2、林志學³

台中榮民總醫院埔里分院 外科部 泌尿科¹

國立中興大學轉譯醫學中心²

弘光科技大學 醫療健康學院 營養系³

Evaluate the effect of oligo-fucoidan prevent renal fibrosis on a mouse model of unilateral ureter obstruction

Tsung-Wei Wang MD^1,3, Chih-Hsueh Lin PhD³

Division of Urology, Departments of Surgery, Puli Branch, Taichung Veterans General Hospital ¹

Rong Hsing Research Center for Translational Medicine, National Chung Hsing University²

Department of Nutrition, College of Medical and health care, Hung Kuang University ³

Purpose:

Ureteral obstruction is one of major causes of chronic kidney disease (CKD). Tubulointerstitial fibrosis is a common appearance of ureteral obstruction induced progressive CKD. Fucoidan, a brown seaweed extracted sulphated polysaccharide, is used as functionally additive food for cancer patients. It is suggested the fucoidan with anti-inflammatory and anti-oxidative effect by several studies in vitro and in vivo. However, fucoidan is effective or not to prevent kidney fibrosis remains unclear. The aim of this study was to evaluate the efficacy of fucoidan in kidney fibrosis using a murine model of reversible unilateral ureteral obstruction(R-UUO).

Materials and Methods:

Fifteen mice were enroll into three study groups, UUO, F-100 and UUO-F-100, randomly and equally. UUO and UUO-F-100 groups recevied unilateral ureteral ligation followed by relief at 5 days after ligation in left side ureter. Fucoidan in 100 mg/kg starts daily oral dosing at 3 days before ureteral ligation and consecutive days until 7 days after relief ureteral ligation for F-100 and UUO-F-100 groups. At the finishing day, all mice were sacrificed by over dose isoflurane followed by cervical dislocation. The blood from inferior vena cava were collected into sampling vials with colt activators. Serum samples were harvested into other clean vials for determination of biological markers such as TGF-. Kidneys were harvested and immerced into 10% buffered formalin. Three to five micro-meters parafilm sections were cutted and stained with hematoxlin and eosin. Pathophysiological signs were inspected and scored by a pathologist.

Results:

In histopathology, compared to non-obstructed right site kidney, severe damages were found in the obstructive left site kidney within UUO or UUO-F-100 mice. There is no difference between right and left site kidney within F-100 mice. The fucoidan treated UUO-F-100 mice had less damage than UUO mice in the left site kidney. There is no significant difference in right site kidney among three groups of this study. It is a trend but without statistical difference in the serum levels of TGF- among groups.

Conclusion:

Our histopathological founding suggests that treatment with fucoidan results less progressively damages of ureteral obstructed kidney in R-UUO murine model.