適體在調節前列腺炎症過程中的作用

陳慧雅、何東儒、陳志碩、林威宇、黃雲慶、林健煇、黃國財、劉昱良

嘉義長庚紀念醫院 外科部 泌尿科

The role of Aptamers in regulation of prostate inflammatory process

Wai-Nga Chan*, Dong-Ru Ho, Chih-Shou Chen, Wei-Yu Lin, Yun-Ching Huang, Jian-Hui Lin, Kuo-Tsai Huang, Yu-Liang Liu

Divisions of Urology, Department of Surgery, Chang Gung Medical Foundation, Chiayi City, Taiwan

Purpose:

We hope to study the effectiveness of DNA aptamers to inhibit IL-1β which can directly attract innate inflammatory cells, cause tissue destruction, altered pain threshold, and initiate adaptive immunity that mediated prostate inflammation in an experimental rat model of non-bacterial prostatitis (NBP).

Materials and Methods:

In this study, we investigated the effects of inflammatory cytokine targeting aptamers (ICTA), a putative neutralizer of TNF-α and IL-1β activation, on local carrageenan-induced prostate inflammation in aged rats. The von Frey filament and tail-flick tests were performed to determine mechanical allodynia (a painful sensation caused by innocuous stimuli, e.g., light touch) and thermal hyperalgesia (a condition of altered perception of temperature), respectively, and prostate inflammation was examined 7 days after the administration of drugs.

Results:

The levels of mononuclear cell infiltration, pro-inflammatory cytokine interleukin-1β, caspase 1(casp-1), and NALP 3 (NALP3) in the prostate of aged rats were increased 168 h after the NBP induction. Treatment with ICTA significantly attenuated carrageenan-induced allodynia, hyperalgesia, the increase in prostate glandular hyperplasia, and immune cell infiltration, and elevated levels of downstream cytokines including IL-18, TNF-α, and IL-33 in aged rats. Apoptosis markers, Bcl-2 and caspase-3, were found elevated.

Conclusions:

Our results showed that ICTA provides protection against local carrageenan exposure-induced enhanced pain sensitivity (allodynia and hyperalgesia), and that the protective effects may be associated with its ability to neutralize carrageenan-induced IL-1β activation, and result in apoptosis in the prostate of aged rats.