K他命膀胱炎之細胞緊密黏附蛋白的基因變化
范綱毅1、張淑貞2、吳勝堂3、程君弘4、蒙恩3*
國防醫學院醫學科學研究所1；成體幹細胞暨組織再生工程實驗室2；三軍總醫院外科部泌尿外科3；國防醫學院生物及解剖學科暨研究所4
The genetic profile variation of cell adhesion molecules in ketamine-induced cystitis
Gang-Yi Fan1, Shu-Jen Chang2, Sheng-Tang Wu3, Juin-Hong Cherng4, En Meng3*
1Graduate Institute of Medical Sciences, National Defense Medical Center, Taipei, Taiwan.
2Lab of Adult Stem cell and Tissue Regeneration, Taipei, Taiwan.
3Division of Urology, Department of Surgery, Tri-Service General Hospital, Taipei, Taiwan.
4Department and Graduate Institute of Biology and Anatomy, Taipei, Taiwan.
Purpose: The pathology of Ketamine Cystitis (KC) is unclear and the lack of effective treatment. The purpose of this study was to investigate the relationship between cell adhesion molecules(CAM) genetic variation and prognosis in KC inflammatory response.
Materials and Methods: The pigs were divided into a control group that received 0.9% saline injection and ketamine injection for 12 weeks. Then the pigs received collagen/alginate treatment for 4 weeks. Blood and bladder tissue samples were collected. Luminex magnetic bead assay was used to detect the cytokines level in blood samples and understand the immune response activity before and after ketamine stimulation.
Results: The gene of cell adhesion molecules (CAMs) expressed in ketamine–induced cystitis pig at 12 weeks and after treated with GAGs for 4 weeks. This result demonstrated that the CAMs activate the leukocytes transendothelial migration (TEM) after GAGs treatment. However, the leukocyte enters the extracellular matrix and under the chemo-attractant gradient reaches the site of inflammation, where it performs its essential for tissue repair after an injury. In other hand, ICAM-1 is critical in recruitment of Tregs to inflamed site, indicated that maintaining peripheral tolerance, through the ability to suppress T cells at all stages of an immune response.
Conclusions: Regulation of cell adhesions during wound repair is important for cell migration, proliferation, and protein production. One important role is in the immune system, in which white blood cells migrate along the connective endothelium following cellular signals to damaged biological tissue. Leukocytes cross blood vessels and extravasation of the endothelial cell layer is an important process for innate or adaptive immune system responds. According to the findings of this study, we can better understand the pathophysiology of KC.