八味地黃丸可以改善大鼠之愷他命膀胱炎
Ba-Wei-Die-Huang-Wan can Ameliorate Ketamine-induced Ulcerative Cystitis in Rats
李偉嘉1，蘇家豪2，田祐霖3，莊燿吉1
高雄長庚紀念醫院泌尿科1，轉譯中心2，兒科部.3
Wei-Chia Lee, M.D. Ph.D. 1, Chia-Hao Su, Ph.D.2, You-Lin Tain, M.D. Ph.D.3, Yao-Chi Chuang, M.D. 1.
Division of Urology1, Institute for Translational Research in Biomedicine3, and Department of pediatrics3, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung, Taiwan.
Purpose: We investigate the effect of Ba-Wei-Die-Huang-Wan on ketamine-induced ulcerative cystitis in rat’s bladder.
Materials and methods: Female Sprague-Dawley rats were subject to three groups, which receive saline, ketamine (25mg/kg/day, i.p.) or ketamine combined with Ba-Wei-Die-Huang-Wan (90mg/kg/day) treatment. Voiding behavior of rats was evaluated by metabolic cage study, and cystometry. Paraffin-embedded sections were stained. Bladder mucosa and muscle proteins were also assessed by Western blotting.
Results: As compared to controls, the ketamine group showed increased urinary frequency, and bladder overactivity, whereas the Ba-Wei-Die-Huang-Wan group did not. The ketamine group had significant protein overexpression in bladder mucosal TRPV1 receptor, and detrusor M2, M3 receptors. Also, the ketamine group had significantly increased inflammatory markers, including TNF-α, and ICAM-1 in detrusor. However, the ketamine/Ba-Wei-Die-Huang-Wan group had insignificant changes from controls.
Conclusion: Ba-Wei-Die-Huang-Wan intake can ameliorate bladder overactivity and hypersensitivity of ketamine-induced ulcerative cystitis of rats by decreasing bladder inflammatory signals of TNF-α, and ICAM-1, and suppressing mucosal TRPV1 receptor as well as detrusor M2 receptors overexpression.