衛生福利部桃園醫院 外科部 泌尿科；1 三軍總醫院病理部，
Androgen-dependent prostate cancer cells into non-androgen dependent process the role of microrna
Jui-Ming Liu, Ren-Jun Hsu 1
Division of Urology , Department of Surgery, Taoyuan General Hospital, Ministry of Health and Welfare, Taoyuan, Taiwan; Department of Pathology and Graduate Institute of Pathology and Parasitology1, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan
To compare the observed androgen-dependent prostate cancer cells (LNCaP), transformed into a differential expression of androgen-independent prostate cancer cells (LNCaP -androgen independent) process microRNA explore high microRNA expression differences in the conversion process regulatory mechanisms may play its target genes.
Materials and Methods:
LNCaP, anti-androgen drugs (high expression and low expression for 3 months LNCaP and LNCaP -androgen independent transformed into microRNA expression between cells, choose large differences in the use of Agilent to validate, and bioinformatics analysis, looking for differences in the expression of microRNA target gene. Luciferase reporter assay to demonstrate the use of the target gene does. This will take advantage of siRNA way to suppress or to increase the performance of high performance differences of microRNA, microRNA to confirm the high performance difference does affect the formation of androgen-independent prostate cancer cells.
Under the gene chip can get more than 100 have differences in microRNA, but we only pick the difference more than 10 times higher than the microRNA analysis to identify prostate cancer cells are most likely to affect the transition from androgen-dependent a non-androgen dependent microRNA. We found miR-221 is the highest performance microRNA.
The miR-221 is one of the highest performance difference does affect the formation of androgen-independent prostate cancer cells.