研究K他命對鼠類膀胱功能隨時間演變所產生的影響
葉忠信1,3、吳宜娜2、廖俊厚3,4,、江漢聲2,,4,5
1新光醫院 外科部 泌尿科, 2輔仁大學 生物醫學暨藥學研究所, 3輔仁大學 醫學系; 4天主教耕莘醫院 外科部 泌尿外科; 5台北醫學大學附設醫院 泌尿科
Time-dependent effects of ketamine on bladder function in rodent model
Chung-Hsin Yeh1,3, Yi-No Wu2, Chun-Hou Liao 3,4, Han-Sun Chiang2,4,5
1Division of Urology, Department of Surgery,Shin Kong WHS Memorial Hospital , Taipei, Taiwan, 2Graduate Institute of Biomedical and Pharmaceutical Science, Fu Jen Catholic University, New Taipei City, Taiwan; 3School of Medicine, Fu Jen Catholic University, New Taipei City, Taiwan ,4Division of Urology, Department of Surgery, Cardinal Tien Hospital; New Taipei City, Taiwan, 5Department of Urology, Taipei Medical University Hospital, Taipei, Taiwan
Purpose:
ketamine abuse affects the urinary bladder, resulting in an increase of urinary frequency, urgency, hematuria, painful micturition, which are related to cystitis; but its pathogenesis remains unclear. The present study was to investigate the impact of ketamine-induced ulcerative cystitis on rat urinary bladder under different time periods after ketamine administration and elucidate potential factors underlying ketamine effects.
Materials and methods:
Thirty-two rats were randomly divided into five equal groups which received saline or high dose ketamine (100mg/kg/day) for a period of 14, 28 and 56 days (d). In each group, cystometrogram was performed and paraffin-embedded sections were stained with Masson’s trichrome stain and conducted transmission electron microscopy (TEM) in the bladder tissue.
Results:
Ketamine-treated rats had increased urinary frequency and reduced voided volume compared to saline-treated rats at 28 d. Histological study showed proliferative epithelium and severe atrophy of detrusor muscle layer in rat bladders at 14 d after the ketamine administration. The barrier of the bladder tissue was affected briefly at 14 d after treatment with ketamine. The ultra-structural analyses revealed increase of autolysosome at 14 d and 28 d and loss of the adherence junction in smooth muscle at 28 d following ketamine treatment. Increased irregularity of the collagen was observed in the detrusor muscle layer at 28 d after administration of ketamine.
Conclusion:
Long-term ketamine administrations affect autolysosome’s numbers of urothelium in rat and mediate the pathological changes of cystitis, and this might play an important role in the pathogenesis of ketamine-associated cystitis.