TLR6 和CTGH 可明顯上調控靜水壓力增強絲裂霉素C對泌尿上皮癌感受性的研究
Toll-like receptor 6 and connective tissue growth factor are significantly upregulated in mitomycin-c-treated urothelial carcinoma cells under hydrostatic pressure stimulation
Shao Kuan Chen
Sijhih Cathay General Hospital
Purpose: Urothelial carcinoma (UC) is the most common histologic subtype of bladder cancer. The administration of mitomycin C (MMC) into bladder after transurethral resection of the bladder tumor (TURBT) is a common treatment strategy for preventing recurrence after surgery. We previously applied hydrostatic pressure combined with MMC in UC cells and found that hydrostatic pressure synergistically enhanced MMC-induced UC cell apoptosis via the Fas/FasL pathways.
Materials and Methods: To understand the alteration of gene expressions in UC cells caused by hydrostatic pressure and MMC, oligonucleotide microarray was used to explore all of the differentially expressed genes.
Results: After bioinformatics analysis and gene annotation, toll-like receptor 6 (TLR6) and connective tissue growth factor (CTGF) showed significant up-regulation among altered genes, and their gene and protein expressions with each treatment of UC cells were validated by quantitative real-time PCR and immunoblotting.
Conclusion: Under treatment with MMC and hydrostatic pressure, UC cells showed increasing apoptosis via extrinsic pathways through upregulation of TLR6 and CTGF.