TLR6 和CTGH 可明顯上調控靜水壓力增強絲裂霉素C對泌尿上皮癌感受性的研究
陳紹寬
汐止國泰醫院
Toll-like receptor 6 and connective tissue growth factor are significantly upregulated in mitomycin-c-treated urothelial carcinoma cells under hydrostatic pressure stimulation
Shao Kuan Chen
Sijhih Cathay General Hospital
 
Purpose: Urothelial carcinoma (UC) is the most common histologic subtype of bladder cancer. The administration of mitomycin C (MMC) into bladder after transurethral resection of the bladder tumor (TURBT) is a common treatment strategy for preventing recurrence after surgery. We previously applied hydrostatic pressure combined with MMC in UC cells and found that hydrostatic pressure synergistically enhanced MMC-induced UC cell apoptosis via the Fas/FasL pathways.
Materials and Methods: To understand the alteration of gene expressions in UC cells caused by hydrostatic pressure and MMC, oligonucleotide microarray was used to explore all of the differentially expressed genes.
Results: After bioinformatics analysis and gene annotation, toll-like receptor 6 (TLR6) and connective tissue growth factor (CTGF) showed significant up-regulation among altered genes, and their gene and protein expressions with each treatment of UC cells were validated by quantitative real-time PCR and immunoblotting.
Conclusion: Under treatment with MMC and hydrostatic pressure, UC cells showed increasing apoptosis via extrinsic pathways through upregulation of TLR6 and CTGF.  
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    發表人
    TUA秘書處
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    台灣泌尿科醫學會
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    討論式海報
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    2015-05-22 16:14:00
    最近修訂
    2015-05-22 16:15:20
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