海綿體神經刺激對大白鼠海綿體內壓、膀胱壓及直腸壓之同步效應
陳光國
振興醫療財團法人振興醫院 泌尿部
The effect of cavernous nerve stimulation on concurrent intracavernous, intravesical and intrarectal pressures in the rat
Kuang-Kuo Chen
Department of Urology, Cheng Hsin General Hospital, Taipei, Taiwan
Purpose: Cavernous nerve (CN) plays a pivotal role in peripheral neural regulation of penile erection. In our previous study, a simultaneous increase of intracavernous pressure (ICP) and seminal vesical pressure (SVP) after electrical stimulation of cavernous nerve was found. We proposed that there may be a retrograde stimulatory impulse transmission from cavernous nerve to major pelvic ganglion (MPG) to induce a significant increase of SVP after electrical stimulation of cavernous nerve. The MPG contains neurons to innervate three pelvic organs- the penis, urinary bladder, and rectum. Therefore, the aim of this study was to investigate the effect of cavernous nerve-stimulation on concurrent ICP, intravesical pressure (IVP) and intrarectal pressure (IRP) in the rat.
Materials and Methods: Male adult Sprague-Dawley rats anesthetized with Zoletil and xylazine were used. A 26-gauge needle, a PE-50 tube and a 8mm-balloon catheter were inserted into the corpus cavernosum, urinary bladder and rectum, respectively to monitor the ICP, IVP and IRP simultaneously. The CN and MPG were electrically stimulated with the stimulus parameters (1-min train of 2-ms pulses, 20 Hz, 5, 7.5 V), respectively and the ICP, IVP and IRP were recorded simultaneously. The ICP, IVP and IRP before and after electrical stimulation were compared by Wilcoxon signed rank test, respectively. A p < 0.05 was considered significant.
Results: There was a significant concurrent increase of CN stimulation-induced ICP from 8.3 ± 1.6 mmHg to 53.7 ± 6.8 mmHg (p = 0.018), IVP from 20.1 ± 2.6 mmHg to 33.4 ± 2.5 mmHg (p = 0.018) and IRP from 8.1 ± 1.9 mmHg to 39.9 ± 4.5 mmHg (p = 0.018) at 7.5 V, respectively. A simultaneous significant increase of MPG stimulation-induced ICP from 9.9 ± 1.6 mmHg to 58.7 ± 6.9 mmHg (p = 0.018), IVP from 20.1 ± 2.1 mmHg to 33.3 ± 3.0 mmHg (p = 0.018) and IRP from 7.3 ± 2.1 mmHg to 30.7 ± 3.3 mmHg (p = 0.018) at 7.5 V, respectively were found.
Conclusions: The results of this study suggest that electrical stimulation of CN and MPG may elicit a significant concurrent increase of intracavernous, intravesical and intrarectal pressures, respectively in the rat.