血小板血漿對高血脂誘發大鼠勃起功能障礙的療效

鄭存翔¹、黃雲慶^1、2、陳志碩¹、何東儒¹

¹嘉義長庚紀念醫院外科部泌尿外科; ²長庚大學醫學院醫學系

Intracavernous Injection of Autologous Platelet-Rich Plasma Ameliorated Hyperlipidemia-Associated Erectile Dysfunction in a Rat Model

Tsun-Hsiang Cheng¹, Yun-Ching Huang^1,2, Chih-Shou Chen¹, Dong-Ru Ho¹

¹Division of Urology, Department of Surgery, Chang Gung Memorial Hospital, Chiayi, Taiwan;

²Department of Medicine, College of Medicine, Chang Gung University, Taoyuan, Taiwan;

Purpose:

yperlipidemia is associated with an increased risk of erectile dysfunction (ED) mediated by endothelial damage. Platelet-rich plasma (PRP) contains numerous angiogenic growth factors. Currently, evidence supporting the use of PRP for ED treatment is limited. Therefore, we investigated PRP in a rat model of hyperlipidemia-associated ED.

Materials and Methods:

Thirty 2-month-old male Sprague-Dawley rats were randomly divided into 3 groups. 20 rats were fed a high-fat diet for 5 months and were randomly divided into 2 groups: (i) rats in the H group received supernatant injection into the corpus cavernosum weekly for 4 weeks; (ii) rats in the H + PRP group received PRP injection into the corpus cavernosum weekly for 4 weeks. 10 rats were fed a standard diet for 5 months and received supernatant injection into the corpus cavernosum weekly for 4 weeks (N group). 7 days after the 4^th injection, all rats underwent erectile function testing and then euthanasia. Erectile function was evaluated by measuring intracavernous pressure (ICP) and mean arterial pressure (MAP). Serum and penile tissue were collected for metabolic variable assessment and histochemical examination, respectively.

Results:

Intracavernous pressure/MAP and area under the curve/MAP ratios were significantly higher in the N and H + PRP groups than in the H group. Insulin-like growth factor-1, brain-derived neurotrophic factor, and vascular endothelial growth factor levels were significantly higher in the H + PRP group than in the N and H groups. Corporal neuronal nitric oxide synthase, endothelial nitric oxide synthase, and endothelial cells were weakly expressed in the H group compared with the N and H + PRP groups. Intracorporal oxidative stress and apoptotic index were significantly higher in the H group than in the N and H + PRP groups.

Conclusions:

This preclinical evidence suggests that clinical trials of PRP in men with ED should be considered. PRP may play a role in ED management.