精子 DNA 片段化比率搭配電腦輔助精液形態運動參數可增益男性生育能力評估

林于巧1林欣達2,吳孟興2,蔡立忠3,陳鈺瀅2,洪貴香2,吳柏萱2,陳達生2,歐凰姿3,4,鄭裕生1

成功大學醫學院附設醫院泌尿部1,成功大學醫學院附設醫院婦產部2成功大學臨床藥理研究所3,成功大學藥學系4

Combining Sperm DNA Fragmentation Index with Computer-Assisted Semen Morphokinematic Parameters as a Better Window into Male Fertility

Yu-Chiao Lin1, Hsin-Ta Lin2, Meng-Hsing Wu2, Li-Chung Tsai3, Yu-Ying Chen2, Kuei-Hsiang Hung2, Po-Hsuan Wu2, Ta-Sheng Chen2, Huang-Tz Ou3,4, Yu-Sheng Cheng1

1Department of Obstetrics and Gynecology, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan

2Institute of Clinical Pharmacy and Pharmaceutical Sciences, College of Medicine, National Cheng Kung University, Tainan, Taiwan

3School of Pharmacy, College of Medicine, National Cheng Kung University, Tainan, Taiwan

4Department of Urology, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan

 

Purpose:

This study aimed to assess the correlation between computer-assisted semen analyzer (CASA) morphokinematic parameters and sperm DNA fragmentation index (DFI), and the diagnostic value for male reproduction by combining semen morphokinematic parameters and DFI.

 

Materials and Methods:

Total fifty male participants were recruited, and all collected semen samples underwent semen analyses and SCD assays. The DFI was defined by the percentage of very small-size halo, without a halo, and degraded sperm cells. At least 500 sperm cells typically need to be evaluated in each specimen to produce the DFI. The predictive ability of CASA parameters and DFI (with different cutoffs, i.e., 15% and 20%) for infertility was assessed using receiver operating characteristic curve (ROC) analysis.

 

Results:

The CASA parameters (i.e., total motility [r =-0.57], progression motility [r= -0.55], rapidly progressive motility [r = -0.55]) were significantly correlated with DFI (p<0.001). The predictive ability of the 15%-cutoff DFI data was better than that of the 20%-cutoff or continuous DFI data.The model comprising the CASA parameters, 15%-cutoff DFI and 4%-cutoff normal morphology had the highest AUC (0.8125) for infertility.

 

Conclusion:

Combining the sperm DFI with CASA parameters might be a better diagnostic tool for male reproduction.

 

Keywords: sperm chromatin dispersion, DNA fragmentation index, computer-assisted semen analyzer, conventional semen analysis, male infertility.

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    台灣泌尿科醫學會
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    2022-06-07 10:03:31
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    2022-06-07 10:04:11
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