#1459

Engineering Biocompatible Solutions for POP: eMSC-Seeded and Plasma-Treated Scaffolds

D. Hennes¹, S. Darzi¹, K. Paul¹, W. Jerome¹, G. Caroline¹, R. Anna², M. Shayanti¹

¹Hudson Institute of Medical Research, Melbourne, Australia
²Monash Health, Melbourne, Australia

Introduction:

Pelvic Organ Prolapse (POP) remains a prevalent condition with limited durable and biocompatible surgical solutions. The withdrawal of non-degradable polypropylene mesh due to safety concerns has prompted the need for innovative bioengineered alternatives. This study evaluates two translational strategies to enhance implant integration and reduce complications: mesenchymal stem cell (MSC) augmentation and plasma polymer surface modification.

Material and methods:

Human endometrial mesenchymal stem/stromal cells (eMSC; SUSD2⁺) were isolated, expanded, and seeded onto nanostructured poly-L-lactide-co-ε-caprolactone (PLCL) scaffolds. An ovine vaginal repair model (approved by Monash Animal Ethics Committee #MMCA-2018) was used to compare native repair, PLCL implants, and PLCL + eMSC constructs. Modified POP-Q scores were recorded pre-op and at 7, 30, and 90 days post-op. Tissue samples were analysed using histology, immunohistochemistry, scanning electron microscopy (SEM), and immunofluorescence to assess tissue integration, inflammation, ECM remodelling, and angiogenesis. For surface modification studies, PLCL scaffolds were treated with plasma polymerised allylamine and implanted into a separate ovine model. Groups included uncoated, freshly coated, and delayed implantation PLCL. Post-op tissue was harvested at day 30 for histological and SEM analysis.

Results:

Stem cell-seeded PLCL scaffolds showed improved integration, minimal inflammatory response, and enhanced collagen and elastin organisation compared to controls. eMSC constructs exhibited histological evidence of angiogenesis and tissue maturation, with limited smooth muscle disruption. Plasma-coated PLCL significantly reduced foreign body giant cell presence and promoted collagen preservation compared to uncoated scaffolds. Delayed implant groups retained favourable integration with less tissue degradation.