尿液中微粒TACSTD2為膀胱癌生物標記
陳建綸 鄧志剛 吳志慶 鍾婷 余兆松 張玉生 張慧朗 陳怡婷
林口長庚醫院 泌尿科 長庚大學
The urinary microparticle tumor-associated calcium-signal transducer 2 as a bladder cancer biomarker
Chien-Lun Chen, Petrus Tang, Chih-Ching Wu, Ting Chung, Jau-Song Yu, Yu-Sun Chang, Phei-Lang Chang, Yi-Ting Chen
Department of Urology, Chang Gung Memorial Hospital, Linko
Chang Gung University, Taoyuan , Taiwan
Purpose: In contrast to PSA for prostate cancer, no reliable bladder cancer biomarker is currently widely applicable for the detection and follow-up of bladder cancer. We employed a strategy combining isotopic dimethylation labeling coupled with liquid chromatography-tandem mass spectrometry (LC−MS/MS) to discover bladder cancer biomarkers in urinary microparticles isolated from hernia (control) and bladder cancer patients.
Materials and Methods: The urine specimens of bladder cancer patients and age-matched hernia patients (n=81) were collected in the morning of surgery. The surgically resected bladder tumors were all pathologically identified and determined into 3 groups for comparison; namely, Low-grade/Early stage (LgEs, n=40), High-grade/Early stage (HgEs, n=63), and High-grade/ Advanced-stage (HgAs, n=37).
Results: A total of 107 proteins out of 2964 proteins were identified in this approach as candidate biomarkers. Differences in the concentrations of 29 proteins were precisely quantified by LC−MRM/MS. There were 24 proteins changed significantly (p<0.05) between bladder cancer and hernia. TACSTD2 concentrations measured by LC-MRM/MS were 6.5-fold higher in bladder cancer urinary microparticles than in hernia urinary microparticles. In raw urine specimens (n = 221) using ELISA, the area-under-the-curve values of TACSTD2 was 0.80.
Conclusion: Our study revealed that TACSTD2 showed strong association with bladder cancer in urine specimens, and thus represents a potential biomarker for noninvasive screening for bladder cancer.