微管聚合促進蛋白基因變異於膀胱癌之角色
林柏宏1、張英勛1、馮思中1、曹李丞1、劉忠一1、莊正鏗1、翁文慧2*
1林口長庚醫院 外科部 泌尿科；2國立台北科技大學 化學工程與生物科技系
TPPP gene alterations AND ITS ROLE in bladder cancer
Po-Hung Lin1,Ying-Hsu Chang1, See-Tong Pang1, Lee-Cheng Tsao1, Chung-Yi Liu1,
Cheng-Keng Chuang1, Wen-Hui Weng2*
1Division of Urology, Department of Surgery, Chang Gung Memorial Hospital,
Chang Gung University, Taiwan.
2Department of Chemical Engineering and Biotechnology, Graduate
Institute of Biotechnology, National Taipei University of Technology, 1, Section 3,
Chung-hsiao East Road, Taipei 10608, Taiwan.
*Corresponding author
Purpose: We studied the gene copy number alteration of TPPP (Tubulin Polymerization Promoting Protein ) in bladder cancer using fluorescence in situ hybridization (FISH) based assay and correlated it’s the result with clinical parameters.
Materials and Methods: Samples and clinical data from patients with bladder cancer and clinical data were collected. TPPP gene was evaluated by FISH in 52 patients. The probe targeting the gene TPPP were established for FISH experiment. At least 300 non-overlapping cells in each case were analyzed by two observers.
Results: We could see different genetic copy number alterations of TPPP in studied sample. Further analysis with the clinical data, the TPPP gene alteration was strongly correlated with tumor grade, in which the amplification of TPPP gene was significantly correlated with high-grade tumor (p<0.001). Besides, the amplification of TPPP gene was positively correlated with tumor stage (p<0.05).
Conclusions: The genetic alterations of TPPP gene were much higher in high-stage and -grade bladder cancer. This result suggests that TPPP may play a role in bladder cancer pathogenesis, and TPPP gene alteration may serve as a biomarker for bladder cancer detection.