不同種類油脂之高脂高果糖飲食對於雄鼠生殖功能之影響
曹智惟1、 林士祥2、 吳勝堂1、 查岱龍1、 劉沁瑜3
1國防醫學院 三軍總醫院 外科部；2台北醫學大學 保健營養學系；3 輔仁大學 營養科學系
The effects of different type oil in high-fat and high- fructose diets on reproduction function in male rats
Chih-Wei Tsao1、 Shyh-Hsiang Lin2、 Sheng-Tang Wu1、Tai-Lung Cha1 and Chin-Yu Liu3
1Division of Urology, Department of Surgery, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan；2 School of Nutrition and Health Sciences, Taipei Medical University, Taipei, Taiwan；3 Department of Nutritional Science, Fu Jen Catholic University, New Taipei, Taiwan
Purpose: The aim of this study was to investigate and compare the effects of high-fructose, high-fat diet supplemented with soy bean oil or coconut, on the testicular testosterone biosynthesis, antioxidant capability and cell apoptosis in male rats.
Materials and Methods: Wistar rats were 24 male, 6 weeks old and divided into 3 groups: control group, soybean oil group and coconut oil group. Soybean oil group and coconut oil group were fed with high fat (39 % of total calorie) high fructose (48 % of total calorie) diets. After 20 weeks, rats were given sacrificed.
Results: The soybean oil group had lowest serum testosterone level and saturated fatty acid ratio in testis fatty acid composition; the expression of Cytochrome P450 (CYP17A1) involved in testosterone biosynthesis and glutathione peroxidase activity was highest among groups. Also enhanced Tumor necrosis factor α (TNF α) and Nuclear factor kappa-light-chain-enhancer of activated B cells (NF κB). Moreover, western blot analysis clearly showed the significantly increased testicular cleavage caspase 3 and PARP (poly ADP ribose polymerase) expression in the soybean group.
Conclusion: These results suggest that high-fructose and high-soybean diet decreased reproductive function due to modify testicular fatty acid composition, increased the oxidative stress in the testis and germ cell apoptosis in male rats.