探討攝護腺癌患者血液中外泌性miRNA作為早期檢測標誌
柳瑞明、許仁駿1
衛生福利部桃園醫院 外科部 泌尿科；1 三軍總醫院病理部，
The serum microrna in prostate cancer patients as a biomarker for early detection
Jui-Ming Liu, Ren-Jun Hsu 1
Division of Urology , Department of Surgery, Taoyuan General Hospital, Ministry of Health and Welfare, Taoyuan, Taiwan; Department of Pathology and Graduate Institute of Pathology and Parasitology1, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan
Purpose:
Prostate cancer is the 6th most cancer death in Taiwan. There are no significant symptoms and signs in early prostate cancer. MicroRNA is stable in the cell or tissue and not easily to be degraded. To identify whether microRNA a potential biomarker for early detection of prostate cancer, we conducted this study.
Materials and Methods:
We collected serum from two different prostate cancer patients. Serum from other four non-prostate cancer patients was collected as control group. The QRT-PCR array (782 micro RNAs) was used to select high specific expressed microRNAs from serum of prostate cancer patient. We used above specific microRNAs to detect serum from 20 prostate cancer patients and 40 non-prostate cancer subjects.
Results:
There were 13 microRNAs highly expressed in serum of prostate cancer patients than control group. After amplification, there were only 2 microRNAs highly correlation with prostate cancer. We overexpressed these 2 microRNAs in LNCaP, increased carcinogenesis in LNCaP was noted.
Conclusions:
The microRNA is a potential biomarker in early detection of prostate cancer