基質金屬蛋白酶-7基因型與中台灣攝護腺癌風險的關聯性

廖丞晞^1,2,3,4 張文馨^2,3 吳錫金⁵ 蔡佳紋^2,3 包大靝^2,3,4

¹國軍臺中總醫院外科部泌尿外科; ²中國醫藥大學生物醫學研究所; ³泰瑞法克斯癌症研究室;

⁴國防醫學院臨床醫學研究所; ⁵中國醫藥大學附設醫院泌尿外科

The Association of Matrix Metalloproteinase-7 Genotypes with Prostate Cancer Risk in mid-Taiwan

Cheng-Hsi Liao^1,2,3,4 , Wen-Shin Chang^2,3 , Hsi-Chin Wu⁵, Chia-Wen Tsai^2,3,and Da-Tian Bau^1,2

¹ Division of Urology, Department of Surgery, Taichung Armed Forces General Hospital, Taichung, Taiwan, R.O.C.;

² Graduate Institute of Biomedical Sciences, China Medical University, Taichung, Taiwan, R.O.C.;

³ Terry Fox Cancer Research Laboratory;

⁴ Graduate Institute of Medical Sciences, National Defense Medical Center, Taipei, Taiwan, R.O.C.;

⁵ Department of Urology, China Medical University Hospital, Taichung, Taiwan, R.O.C.;

Purpose: Prostate cancer is one of the most commonly diagnosed malignancies among males worldwide. It has been shown that MMP-7 gene is closely correlated with prostate carcinogenesis. However, the role of the MMP-7 genotypes has been seldom examined among prostate cancer patients. Therefore, the purpose of the study was to evaluate the contribution of MMP-7 promoter genotypes A-181G (rs11568818) and C-153T (rs11568819) to prostate cancer risk in Taiwan.

Materials and Methods: Two hundred and eighteen prostate cancer patients and 436 sex- and age-matched healthy controls were genotyped for MMP-7 rs11568818 and rs11568819 by polymerase chain reaction-restriction fragment length polymorphism and direct sequencing methodologies.

Results: The percentages of wild type AA, and variant AG and GG genotypes on MMP-7 rs11568818 were 85.3, 13.5, and 1.2% among the prostate cancer cases and 87.6, 10.1, and 2.3% among the healthy controls, respectively (p for trend=0.2557). Interestingly, no MMP-7 rs11568819 genotypes were identified among Taiwanese. The allelic frequency distribution also showed that the variant G allele of MMP-7 rs11568818 seemed not to be a determinant of prostate cancer risk (p=0.7977). There was no joint effect between the genotypes of MMP-7 rs11568818 and age and smoking status on prostate cancer risk.  

Conclusions: Our findings suggest that rs11568818 and rs11568819 at MMP-7 promoter region, played no role in determining personal susceptibility to prostate cancer in mid-Taiwan.