輻射劑量的不同對於基因和疾病的影響
吳翊豪1、吳世欣2、林承家1
基隆長庚紀念醫院 外科部 泌尿科1；中國醫藥大學生物醫學研究所2
The potential effect of different doses of ionizing radiation on genes and disease
Yi-Hao Wu1, Lawrence Shih-Hsin Wu2, Cheng-Chia Lin1
Department of Urological Surgery, Chang Gung Memorial Hospital, Keelung, Taiwan1 and Graduate Institute of Biomedical Sciences, China Medical University, Taichung, Taiwan2
Purpose: Radiation exposure was usually happened while retrograde intrarenal surgery (RIRS) and Percutaneous nephrolithotomy. The chromosomal aberrations induced by radiation appear about non-randomly distributed across the whole genome. Previous studies have shown that chromosomes with high DNA content are less frequently involved in the formation of symmetrical translocations and dicentric chromosomes than expected, whereas smaller chromosomes are more frequently involved. We hypothesized that these translocation regions are linked to radiation sensitivity.
Materials and methods: We investigated the frequencies of chromosome translocations induced by radiation exposure and adjusted the results according to chromosome length. We specifically analyzed whole blood samples from three participants. The samples were irradiated using 60Co at doses of 0.5, 1, 2.5, and 5 Gy. Traditional Giemsa-trypsin-Wright (GTW)-band staining was performed to identify the translocations in the chromosomes, and results were compared with microarray data generated in our previous study.
Results: Our analysis indicated that chromosomes 9q were the most sensitive to translocations after various doses of radiation, and such translocations occurred in the euchromatin region. Chromosomes 1, 9, 15, and 17 were more sensitive to radiation doses of 0.5 Gy. This observation could be useful when selecting sensitive reference chromosomes in the low dose region. The results of expression profiling analysis for radiation-sensitive regions were similar to those of chromosome translocation analysis.
Conclusion: This study shows that some chromosomes or genomic regions are more sensitive to alteration by radiation exposure.