K他命可以改善老鼠因社交壓力所導致的膀胱失能
陳義昇1, 張尚仁1, 張希賢1, 謝政興1, 楊緒棣1,
1台北慈濟醫院外科部泌尿科
Ketamine ameliorates the social stress related bladder dysfunction in mice
Yi-Sheng Chen1, Shang-Jen Chang1, Hsi-Hsien Chang1, Cheng-Hsing Hsieh1, Stephen Shei-DeiYang1
1 Division of Urology, Taipei Tzu Chi Hospital, New Taipei, Taiwan
Purpose: Chronic social stress (SS) is associated with urinary bladder dysfunction. The aim of the study is to investigate whether ketamine could ameliorate social stress (SS) related bladder dysfunction in mice.
Materials and Methods: Male 5-week FVB mice were randomized to receive or not to receive SS manipulation (SS1 or SS0). The SS0 were then allocated to single or no injection of ketamine (SS0K1 and SS0K0). In the group of SS1, individual FVB mouse was exposed to aggressive male-retired C57BL/6 directly/indirectly for 60 minutes per day for 4 weeks. At day 22, the SS1 mice were allocated to receive single injection of saline (SS1K0), single dose (SS1K1) or 5-day dose (SS1K5) injection of ketamine (25 mg/kg/day/ip). At Day 29, the urinary bladder was isolated and cut into strips and mounted in the organ bath for measurement of detrusor activity. Serum and urine level of brain-derived-neurotrophic factor (BDNF) were measured with ELISA.
Results: As compared to SS0K0, SS0K1 did not differ in voiding frequency and voided volume. SS1K0 had decreased voiding frequency and increased voided volume, which were reversed in SS1K1 and SS1K5. As compared to SS0K0, SS1K0 had higher serum, but not urine, levels of BDNF. The higher serum, but not urine, level of BDNF in SS1K0 was statistically lowered in SS1K1. Detrusor overactivity and impaired detrusor contractility were observed in all SS1 groups, which were not reversed by ketamine.
Conclusions: Short-term administration of ketamine may improve social stress related infrequent voiding and elevated serum BDNF level. However, ketamine did not ameliorate SS related bladder dysfunction.