PD3-6: Extracorporeal shock wave markedly alleviates radiation-induced chronic cystitis in rat
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  • 2019-01-03,
  • 上傳者: TUA秘書處,
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體外震波可顯著緩解大鼠的放射性慢性膀胱炎
吳彥廷, 陳彥達
高雄長庚紀念醫院外科部泌尿科, 高雄, 台灣
 
Extracorporeal shock wave markedly alleviates radiation-induced chronic cystitis in rat
Yen-Ting Wu, Yen-Ta Chen
Division of Urology, Department of Surgery, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung 83301, Taiwan
 
Purpose:
This study tested the hypothesis that extracorporeal shock wave (ECSW) treatment can effectively inhibit radiation-induced chronic cystitis (CC).
 
Materials and Methods:
Adult male Sprague-Dawley (SD) rats (n = 24) were randomly divided into group 1 (normal control), group 2 (CC induced by radiation with 300 cGy twice with a four-hour interval to the urinary bladder), group 3 [CC with ECSW treatment (0.2 mJ/mm2/120 impulses/at days 1, 7, and 14 after radiation)]. Bladder specimens were harvested by day 28 after radiation.
 
Results:
By day 28 after radiation, the degree of detrusor contraction impairment was significantly higher in group 2 than that in groups 1 and 3, and significantly higher in group 3 than that in group 1 (P<0.0001). The urine albumin concentration expressed an opposite pattern compared to that of detrusor function among the three groups (P<0.0001). The bladder protein expressions of inflammatory (TLR-2/TLR-4/IL-6/IL-12/MMP-9/TNF-α/NF-κB/RANTES/iNOS) and oxidative-stress (NOX-1/NOX-2/oxidized protein) biomarkers exhibited a pattern identical to that of urine albumin in all groups (all P<0.0001). The cellular expressions of inflammatory (CD14+/CD68+/CD74+/COX-2/MIF+/substance P+) and cytokeratin (CK13+/HMW CK+/CK+17/CK+18/CK+19) biomarkers, and collagen-deposition/fibrotic areas as well as epithelial-damaged score displayed an identical pattern compared to that of urine albumin among the three groups (all P<0.0001).
 
Conclusion:
ECSW treatment effectively protected urinary bladder from radiation-induced CC.
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