病毒基因型是否能決定濕疣好發位置或其外型
陳偉寶醫師、 吳驩謙博士*
陳偉寶泌尿科診所 知愛堂生物科技股份有限公司
Clinical significance of hpv genotype,prefertial location or shape of condyloma?
Wai Pou Chan Rich Wu*
Chan’s Urologic Clinic *Knowledge-care bioscience Inc.
Purpose: To identify the role of HPV genotypes in pathogenesis of condyloma. Doesn’t it lead to preferential location in human body or special shape of condyloma ?
Material and Method: All patients with problems of condyloma were advised to take HPV DNA check. Procedure performed under patient’s permission. Sampling brush was sent to laboratory for HPV DNA analysis by COBAS HPV 4800 automatic system, which contained Roche LINEAR ARRAY HPV Genotyping Test system for 37 known genotypes. Specimen of un-determined type will run auto-sequencing method. Sequence alignments were obtained by Genbank’s on-line BLAST server.
RESULTS: From Jan.2006 to Dec. 2012, there were 1296 fresh patient with recognizable condyloma. 694 of them were mono-infection (only one HPV genotype identified from lesion). 602 of them were multi-infection ( 2 to 6 genotypes identified from lesion). Leading 8 types of mono-infection: type 6 in 500 cases, 40 in 33, 44 in 16, 42 in 15, 52 in 15, 58 in 14, 16 in 11, and type 51 in 10 cases. Analysis of mono-infection cases as follow:
|
|
M
|
F
|
Age
|
Location (%)
|
Shape (%)
|
|
Low risk type
|
440
|
155
|
33.9±8.1
|
m 26.4,s 60.2,ms,13.4
|
m 7.1, n 62.4, p 25.0,g 5.5
|
|
High risk type
|
42
|
47
|
31.3±6.4
|
m 49.4,s 44.9,ms 5.6
|
m 14.6,n 52.8,p 31.5,g 1.1
|
* Location: m=mucosa, s=skin, ms=mucosa + skin
* Shape: m=macular, n=nodular, p=papillary, g=giant type
CONCLUSION:
1. All HPV identified were belonging to alpha-HPV, according to International Committee of Taxonomy of Virus (ICTV 2002). Groups of Β, γ, ε, mupa. etc are not found yet.
2. Condyloma in a contagious disease, initial location of lesion determined mostly by contact area during sexual behavior, rather than HPV genotype.
3. Shape and size of condyloma seems to be a balance of HPV virulence and host immunity. High risk type condyloma tend to be a broad base lesion but low risk type tend to be a cauliflower lesion.