薑黃素可改善低碳水化合物飲食誘導之雄性代謝功能障礙小鼠的生殖功能

曹智惟¹ 柯佩珊² 楊欣怡² 張庭嘉² 劉沁瑜²

¹國防醫學院 三軍總醫院 外科部 泌尿外科²輔仁大學 營養科學系

Curcumin retrieved reproductive function in male mice with low-carbohydrate-diet-induced metabolic dysfunction

Chih-Wei Tsao¹, Pei-Shan Ke², Hsin-Yi Yang², Ting-Chia Chang² and Chin-Yu Liu²

¹Division of Urology, Department of Surgery, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan

²Department of Nutritional Science, Fu Jen Catholic University, New Taipei, Taiwan

Purpose:

The aim of this study was to investigate the effects of curcumin supplementation on low- carbo-hydrate- diet-induced metabolic dysfunction, testicular antioxidant capacity, apoptosis, inflammation and spermatogenesis in male mice.

Materials and Methods:

Male C57BL/6 mice were fed a normal diet (AIN-93M group, n=12) and a low carbohydrate diet low-carbohydrate diet for 12 weeks (LC group, fed with low carbohydrate diet low-carbohydrate diet, n=48) for 12 weeks, and mice randomly chosen from the LC group were later kept fed their original diet (LC group, n=12). This diet was changed into AIN-93M feed (LC/AIN-93M group, n=12), or a ketogenic diet (LC/KD group, n=12), or a ketogenic diet treated with curcumin supplementation for the final 6 weeks (LC/KDCu group, n=12) for the final 6 weeks.

Results:

A poor sperm morphology and mean testicular biopsy score (MTBS) were observed in the LC and LC/KD groups, but they were eliminated by the normal diet or ketogenic diet with curcumin. The LC group exhibited a lower testicular testosterone level and a lower 17β-HSD activity and protein expression. It This also enhanced apoptosis protein expressions in testis tissue, including Bax/BCl2, cleaved caspase 3, PARP and NF-κB. Meanwhile, we found a statistically significant increase in lipid peroxidation and decreased superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase levels in the LC group.

Conclusion:

Our study indicated that a replacement of with a normal diet or ketogenic diet supplemented with curcumin attenuated the poor semen quality and reduced testosterone levels by the LC diet via by reducing oxidative stress.