以微脂體包覆肉毒桿菌素進行膀胱灌注可以改善大鼠之愷他命膀胱炎
李偉嘉1、田祐霖2、莊燿吉1
高雄長庚醫院泌尿科1, 兒科醫學部2
Bladder instillation of liposome-encapsulated botulinum toxin can ameliorate ketamine-induced ulcerative cystitis in rats.
Wei-Chia Lee, M.D. Ph.D. 1, You-Lin Tain, M.D. Ph.D.2 , Yao-Chi Chuang, M.D. 1*
Division of Urology1 and Department of pediatrics2, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung, Taiwan.
Purpose: We investigate the effect of liposome-encapsulated botulinum toxin (Lipotoxin) on ketamine-induced ulcerative cystitis in rat’s bladder.
Materials and methods: Female Sprague-Dawley rats were subject to three groups, which receive saline, ketamine or ketamine combined with Lipotoxin treatment. Voiding behavior of rats was evaluated by fMRI, metabolic cage study, and cystometry. Paraffin-embedded sections were stained. Bladder mucosa and muscle proteins were also assessed by Western blotting.
Results: As compared to controls, the ketamine group showed high intensity signal on periaqueductal gray, increased urinary frequency, and bladder overactivity, whereas the ketamine/lipotoxin group did not. The ketamine group had significant protein overexpression in bladder mucosal TRPV1 receptor, and detrusor M2, M3 receptors. Also, the ketamine group had significantly increased inflammatory markers, including TNF-α, NF-κB, and COX2 in detrusor. However, the ketamine/lipotoxin group had insignificant changes from controls, except the overexpression in detrusor M3 receptor.
Conclusions: Lipotoxin bladder instillation can ameliorate bladder overactivity and hypersensitivity of ketamine-induced ulcerative cystitis of rats by decreasing bladder inflammatory signals of TNF-α, NF-κB, and COX2, and suppressing mucosal TRPV1 receptor as well as detrusor M2 receptors overexpression.