薑酮奈米粒子對上尿路上皮癌細胞之影響
楊士明1,4、葉碧雯2,3、孔美蘭4、李威明2,3,5、戴明泓1、謝淑貞4*、吳文正2,3,6*
國立中山大學 生物醫學研究所1，高雄醫學大學 泌尿科2，高雄醫學大學附設醫院 泌尿科3，國立中山大學 化學系4 ， 衛福部屏東醫院 泌尿科5，高雄市立大同醫院 泌尿科6
The effects of nanoparticlized zingerone on upper-tract urothelial carcinoma cells
Shih-Ming Yang1,4, Bi-Wen Yeh2,3, Mei-Lang Kung4, Wei-Ming Li2,3,5, Ming-Hong Tai1, Shuchen Hsieh4*, Wen-Jeng Wu2,3,6*
Institute of Biomedical Sciences, National Sun Yat-sen University, Kaohsiung1; Department of Urology, School of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung2; Department of Urology, Kaohsiung Medical University Hospital, Kaohsiung3; Department of Chemistry, National Sun Yat-sen University, Kaohsiung4; Department of Urology, Ministry of Health and Welfare Pingtung Hospital, Pingtung5; Department of Urology, Kaohsiung Municipal Ta-Tung Hospital, Kaohsiung Medical University, Kaohsiung6
Purpose: Nanoparticlized nature compounds have been explored and examined for their anti-cancer efficiency. In this study, we investigate the potential effects of nanoparticlized zingerone on upper-tract urothelial carcinoma (UTUC) cells.
Materials and Methods: The effects of nanoparticlized zingerone on BFTC909 cell line were carried out by MTT assay, colony formation assay, and flow cytometry cell cycle analysis. Akt signaling and apoptotic enzymes were also examined by Western blotting analysis. The corresponded concentration of native compound was assayed and compared.
Results: Nanoparticlized zingerone suppressed cell viability and colony formation ability, and resulted in cell cycle arrest on BFTC909 cell line. Western blotting analysis indicated that it suppressed the activation of Akt, and increased the activities of apoptotic enzymes including caspase-9, -3, and poly (ADP-ribose) polymerase (PARP). As compared to its native compound, nanoparticlized zingerone showed a more powerful effect in suppress the UTUC cells viability.
Conclusions: Nanoparticlized zingerone increased the bio-sensitivity as compared to its native compound on BFTC909 cells in both dose-dependent and time-dependent manners through higher efficiency in inactivation of Akt and increased levels of apoptotic enzymes. It may be potential to utilize in the treatment of UTUC patients with the conjunction of current treatment modalities.