Reversine透過活化AMPK訊息傳遞引起泌尿上皮細胞癌自噬死亡
張舜凱1、沈正煌1、方瓊瑤2
嘉義基督教醫院 1外科部泌尿科，2轉譯醫學研究中心
Reversine induces autophagic cell death through the AMPK pathway in urothelial carcinoma cells
Shun-Kai Chang1, Cheng-Huang Shen1, Chiung-Yao Fang2
1Department of Urology, Chiayi Christian Hospital, Chiayi, Taiwan, ROC
2Department of Medical Research, Chiayi Christian Hospital, Chiayi, Taiwan, ROC
Purpose: Urothelial carcinoma is one of the most common malignancies of the urinary tract. Effective treatment of advanced urothelial carcinoma remains a clinical challenge with poor outcomes in these patients. Previous reports have demonstrated that the expression of aurora kinase is associated with clinical stage and prognosis; hence, aurora kinases are potential targets in urothelial carcinoma therapy.
Materials and Methods: Reversine, an aurora kinase inhibitor, was analyzed for its cytotoxicity in this study. Cell proliferation, flow cytometry, western blotting, and immunofluorescent assay were used to determine the effect of reversine on urothelial carcinoma cells.
Results: The results demonstrated that reversine significantly inhibits the growth of urothelial carcinoma cell lines. Reversine induced cell cycle arrest at the G2/M phase, leading to autophagic cell death by activating the AMPK pathway.
Conclusions: Reversine induced significant cell death in urothelial carcinoma cells. Our results suggest that reversine may be a suitably small molecule for treating urothelial carcinoma in the future.