靜電紡絲素蛋白基質及膀胱脫細胞基質行膀胱替代的比較研究
宋魯傑1§、黃建文1、徐月敏1＊、李赵波2、Sean V. Murphy3、劉陽4、趙維新3、張心如1、劉強強2、朱衛東1、傅強1、張耀鵬2§
1 上海交通大學附屬第六人民醫院泌尿外科，上海，200233，中國；
2 東華大學纖維材料改性國家重點實驗室，東華大學材料科學與工程學院，上海，201620，中國；3 威克弗裡斯特大學再生醫學研究所，威克弗裡斯特大學健康科學系，北卡羅來納州溫斯頓賽勒姆市醫療中心大道，27157，美國；4 濰坊醫學院附屬濰坊市人民醫院泌尿外科，山東濰坊，261042，中國
Tissue Performance of Bladder following Stretched Electrospun Silk Fibroin Matrix and Bladder Acellular Matrix implantation in a Rabbit Model
Lu-Jie Song1§、Jian-Wen Huang1 ＊、Yue-Min Xu1 ＊ 、Zhao-Bo Li2、Sean V. Murphy3、Yang Liu4、Weixin Zhao3、Xin-Ru Zhang1、Qiang-Qiang Liu2、Wei-Dong Zhu1、Qiang Fu1、Yao-Peng Zhang2§
1 Department of Urology, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, Shanghai, 200233, China；2 State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Materials Science and Engineering, Donghua University, Shanghai, 201620, China；3 Wake Forest Institute for Regenerative Medicine, Wake Forest University Health Sciences, Medical Center Blvd, Winston Salem, North Carolina, 27157, USA；4 Department of Urology, Weifang Medical University Affiliated Weifang People’s Hospital, Weifang, Shandong, 261042, China
§Correspondence to: Lu-Jie Song, MD., PhD. Department of Urology, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, 600 Yishan Road, Shanghai 200233, China. Tel: +86-21-64369181; Fax: +86-21-64701361. Email: ljsong@sjtu.edu.cn
Objective: To investigate the tissue performance of bladder following stretched electrospun silk fibroin matrix （SESFM）implantation compared to bladder acellular matrix (BAM) in a rabbit model.
Materials and methods: Novel SESFM and conventional BAM were prepared, and their porosity and pore size distribution were measured. SESFM and BAM were separately transplanted into opposite walls of the bladder of 30 rabbits after stripping the bladder mucosa and smooth muscle (1.5×2.0 cm). At 2, 4 and 8 weeks post-op, the animals (ten rabbits at each time point) were euthanized. The tissue engineered bladder constructs were then assessed by gross anatomical observation, and histological analysis and muscle contractility studies were performed to evaluate tissue regeneration at both matrix implant sites.
Results: SESFM has higher porosity and larger pore size compared with BAM (P< 0.05). At 2 weeks, the presence of vesical calculus was evident in 7/10 rabbits. Histological analysis showed that SESFM and BAM promoted similar degree of urothelium regeneration (P>0.05), and SESFM promoted a higher degree of smooth muscle and vessel regeneration compared to BAM at each time point (P< 0.05). In addition, muscle strips supported by SESFM displayed higher contractile responses to carbachol, KCl, and phenylephrine compared with BAM. At 8 weeks, both matrices elicited similar mild acute and chronic inflammatory reactions.
Conclusion: Our results demonstrated that SESFM has greater ability to promote bladder tissue regeneration with structural and functional properties compared to BAM, and with similar biocompatibility.
Keywords: silk fibroin, bladder acellular matrix, electrospinning, stretching, bladder reconstruction