血小板濃厚血漿透過調節細胞CXCL5改善海綿體神經損傷的勃起功能障礙
吳宜娜1、廖俊厚2,3、吳建志4,5、江漢聲1,2,5
輔仁大學 生物醫學暨藥學研究所1 ; 天主教耕莘醫院 外科部 泌尿外科2; 輔仁大學 醫學系3; 台北醫學大學 醫學系4; 台北醫學大學附設醫院 泌尿科5
Therapeutic efficacy of platelet rich plasma on cavernous nerve injury induced erectile dysfunction may partly mediated by action of cytokine CXCL5.
Yi-No Wu1, Chun-Hou Liao 2,3, Chien-Chih Wu4,5, Han-Sun Chiang1,2,5
Graduate Institute of Basic Medicine, Fu Jen Catholic University, New Taipei City, Taiwan1; Division of Urology, Department of Surgery, Cardinal Tien Hospital2; College of Medicine, Fu Jen Catholic University3 ; School of Medicine, Taipei Medical University, Taipei, Taiwan4; Department of Urology, Taipei Medical University Hospital, Taipei, Taiwan5
Purpose: To explore the potential neurotrophic and tissue-protective factors in platelet-rich plasma (PRP) and evaluate the therapeutic applications of the cytokine identified from PRP in a cavernous nerve (CN) injury rat model.
Materials and Methods: Thirty-eight rats were divided into five groups: group 1 - sham group, group 2 - vehicle-only group, group 3 - 400 µL PRP, group 4 - 100 ng/kg CXCL1 group, group 5 - 30 ng CXCL5. Groups 2–4 were subjected to bilateral cavernous nerve crushing (BCNC) injury. Four weeks later, erectile function was assessed by CN electrostimulation, and the CNs as well as the penile tissue were collected for histological assay. The CNs as well as penile tissue were collected for histologic evaluation and mRNA expression.
Results: found that the PRP contained higher levels of CXCL1, CXCL5, and CCL2. CXCL1 and CXCL5 were expressed strongly and CXCR2 was expressed weakly in the surface of the neurons of the major pelvic ganglion. The intracavernous injection of PRP may promote the stable expression of CXCR2 and increase the expression of CXCL5 and CXCL1 in the major pelvic ganglion after BCNC, and enhance the ability of neurogenic recovery. We found that the intracavernous injection of CXCL5 promoted recovery from CN injury-induced erectile dysfunction by preventing smooth muscle atrophy.
Conclusions: PRP’s therapeutic efficacy on CN injury induced erectile dysfunction may be a synergistically action with other multiple biomolecules and partly mediated by action of cytokine CXCL5.