鐵補充對雄性缺鐵大鼠生殖功能之影響
曹智惟1 吳勝堂1 查岱龍1 劉沁瑜2
1國防醫學院 三軍總醫院 外科部 泌尿外科2輔仁大學 營養科學系
Effects of iron supplement on reproductive function in male rats with iron deficiency
Chih-Wei Tsao1, Sheng-Tang Wu1, Tai-Lung Cha1 and Chin-Yu Liu3
1Division of Urology, Department of Surgery, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan
2Department of Nutritional Science, Fu Jen Catholic University, New Taipei, Taiwan
Purpose:
The aim of the study is to investigate the effects of iron supplement on testicular testosterone biosynthesis, spermatogenesis and apoptosis in male rat with iron deficiency.
Materials and Methods:
Three-week-old Wistar male rats were randomly divided into two groups: iron- adequate control group (AI group, 35 ppm FeSO4) and iron deficiency group (≦ 5 ppm FeSO4), after three weeks, and iron deficiency group was divided into iron-deficient group (ID group, ≦ 5 ppm FeSO4) and five groups: fed diets containing different doses of ferrous sulfate (6, 12, 18, 24 and 35 ppm FeSO4).
Results:
Serum testosterone concentrations of ID group are significantly lower than the AI group, and found that iron supplement can improve incomplete testicular tissue caused by iron deficiency. And in testosterone biosynthesis pathway, iron supplement will increase 3-beta-hydroxysteroid dehydrogenase (3β-HSD) and 17-beta-hydroxysteroid dehydrogenase (17β-HSD) protein expression. Iron deficiency will decrease antioxidant enzyme superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT), and increase cleaved caspase-8 and caspase-3 protein expression. When given more than 12 ppm FeSO4 supplement in iron deficiency male rats, oxidative stress and apoptosis-related protein expression could be decreased .
Conclusion:
Iron supplement could regulate the testicular testosterone biosynthesis and spermatogenesis in iron deficiency male rats.