雷公藤內酯經由關鍵的NF-κB依賴性途徑抑制DU145前列腺癌細胞的遷移和侵襲
廖丞晞1,2,3,4 張文馨2,3 蔡佳紋2,3 吳錫金5 包大靝2,3,4
1國軍台中總醫院外科部泌尿外科 2中國醫藥大學生物醫學研究所 3Terry Fox癌症研究室 4國防醫學院臨床醫學研究所 5中國醫藥大學附設醫院泌尿外科
Triptolide Suppressed Migration and Invasion of DU145 Prostate Cancer Cells via the Critical NF-kappaB-dependent Pathway
Cheng-Hsi Liao1,2,3,4, Wen-Shin Chang2,3, Chia-Wen Tsai2,3, Hsi-Chin Wu5, and Da-Tian Bau2,3
1Division of Urology, Department of Surgery, Taichung Armed Forces General Hospital, Taichung, Taiwan, R.O.C.; 2Graduate Institute of Biomedical Science, China Medical University, Taichung, Taiwan, R.O.C.; 3Terry Fox Cancer Research Laboratory; 4Graduate Institute of Medical Sciences, National Defense Medical Center, Taipei, Taiwan, R.O.C.; 5Department of Urology, China Medical University Hospital, Taichung, Taiwan, R.O.C.;
Purpose: Prostate cancer is the most common male cancer all over the world. Triptolide, one of the components from the Traditional Chinese Medicine Tripterygium wilfordii, has been shown to induce cell death of many human solid tumor cells such as breast, lung, pancreatic, bladder, cervical and colorectal cancers. But its effects on prostate cancer cell have never been revealed in aspects of proliferation or apoptosis, not to mention on migration and invasion capacities. The information prompts us to evaluate the potential anticancer efficacy of triptolide in the prevention and therapy of Taiwan prostate cancer.
Materials and Methods: In the first step, we have examined and revealed the mechanisms of proliferation inhibition and apoptosis induction of triptolide in prostate cancer cell lines, DU145 and PC3. In addition, we have also examined the metastasis capacity of these prostate cancer cell lines via wound scratch, wound healing, transwell, matrigel invasion assays, and revealed the involved signaling pathways and molecules.
Results: In summary, in the DU145 and PC3 prostate cancer cell lines, finding that triptolide suppressed the overall cell survival of DU145 and PC3 prostate cancer cells. In addition, we have found that the mechanism may be related to induction of cancer cell apoptosis, not to simply proliferation.
Conclusion: We have found NF-kappaB-dependent pathway critically involved in triptolide’s inhibition of migration and invasion.