血小板豐富血漿的治療效果應用在鏈脲佐菌素誘導糖尿病大鼠勃起功能障礙
林宛穎、曾筱雯、1吳宜娜、2江漢聲
輔仁大學 醫學院 1醫學系，2生醫藥學所
Therapeutic effects of platelet rich plasma on erectile dysfunction in streptozotocin-induced diabetic rats
Wan-Ying Lin, Hsiao-Wen Tseng, Yi-No Wu1, Chiang HS2
School of Medicine1 and Graduate Institute of Basic Medicine2, College of Medicine, Fu-Jen Catholic University, New Taipei City, Taiwan
Purpose: Radical prostatectomy causes erectile dysfunction (ED) and irreversible morphologic changes in the penis, including induction of endothelial and smooth muscle cells apoptosis in the corpus cavernosum. Platelet rich plasma (PRP) derived from whole blood, centrifuged to remove red blood cells. Recent studies indicated that PRP contains an abundance of growth factors, which enhance wound healing quality and rate. In this study, we investigate the effect of PRP on erectile dysfunction in streptozotocin-induced diabetic rats.
Materials and Methods: 40 of 6-week-old SD male rats received intraperitoneal (IP) injection of STZ (65mg/kg), and 12 weeks later, that were randomized into three groups: control, vehicle-only, PRP treatment. Rats in the PRP treatment and vehicle-only groups were subjected to bilateral cavernous nerve (CN) injury before intracavernosal (IC) injection of PRP or vehicle, respectively.
Four weeks after surgery, erectile function was assessed by measuring intracavernosal pressure (ICP) and other functional parameters of erectile function. Penile tissues were histomorphometrically analyzed for the expression of neural nitric oxide synthase, α-smooth muscle actin and further perform ultrastructural analysis of the corpus cavernosum.
Results: IC injections of PRP increased all erectile function parameters at 28 day post-injury. Ultrastructural analysis revealed the tissue-protection of PRP is through maintaining the structure of adherens junctions to keep integrity the corpus cavernosum.
Conclusions: PRP treatment restored erectile function in a rat model of bilateral CN injury through preservation of smooth muscle cells in the corpus cavernosum. These findings demonstrate that IC injection of PRP improves erection function in STZ-induced diabetic rat. Our study provides a foundation for clinical trials of ED therapy.