利用定量拉曼光譜偵測體外震波碎石手術前病人尿中結晶與微結石的相關性
邱逸淳1,5、陳柏安2,3、江惠華2,3,4、邱文祥1,5
臺北市立聯合醫院忠孝院區 外科部 泌尿科1；國立陽明大學生醫光電研究所2；國立陽明大學醫學工程研究所3；國立陽明大學生醫光電與分子影像研究中心4；國立陽明大學醫學院醫學系5
Correlation between urine crystals and micro stones detected by quantitative micro-raman spectroscopy before and after the eswl
Yi-Chun Chiu1,5, Po-An Chen2,3, Huihua Kenny Chiang2,3,4, Allen W. Chiu1,5
Divisions of Urology, Department of Surgery, Zhongxiao Branch, Taipei City Hospital1
Institute of Biophotonics, National Yang-Ming University2
Institute of Biomedical Engineering, National Yang-Ming University3
Biophotonics and Molecular Imaging Research Center (BMIRC), National Yang-Ming University4
School of Medicine, National Yang-Ming University5
Purpose
With the improvement of the stone management, less stone fragments could be collected after minimalinvasive management, especially after the extracoporeal shock wave lithotripysy (ESWL). But stone analysis still highly involves the further metabolic study and stone management policy. We tried to apply the micro-Raman spectroscopy(MRS) to quantitatively detect the urine crystals and the micro stones in the urine before and after the ESWL to make the correlation between them .
Materials and Methods
In the patients who received the ESWL, urine was collected as one shot in the morning and another one shot ( within 1 hour ) before and after the ESWL on the same day. Then we compared the result of the urine crystal types in the Pre-ESWL urine samples to the subsequently expelled micro urine stones in the Post-ESWL urine samples. All the urine crystal and micro urine stones were analyzed by the MRS-based method.
Results
40 patients with subsequent stone expelling were included in the study. Post-ESWL urine samples from these patients were analyzed, including 26 single composition samples, 9 binary composition samples, and 5 ternary composition samples. There are six COM/COD samples, two COM/Uric acid samples, and one COM/HAP sample. Pre-ESWL urine crystal analysis revealed 86% correlation with the analysis of stone composition. In addition, the crystal type results showed a high correlation, more than 80%, between one shot morning Pre-ESWL urine samples and the post-ESWL urine samples.
Conclusion
This research successfully applied the quantitative MRS-based analysis technique, from bench to bedside, to measure the micro stone components in urine after the ESWL and proved high correlation between urine crystal in the one shot morning urine before the ESWL and post-ESWL micro stones.