雙側海綿體神經損傷大鼠模式證實血小板濃厚血漿透過神經與組織保護效果改善神經性勃起功能障礙
吳宜娜1、廖俊厚2,3、吳建志4,5、江漢聲1,2,5
輔仁大學 基礎醫學研究所1 ; 天主教耕莘醫院 外科部 泌尿外科2; 輔仁大學 醫學系3; 台北醫學大學 醫學系4; 台北醫學大學附設醫院 泌尿科5
Both of the neuroprotective and tissue-protective effects of platelet-rich plasma on erectile function in bilateral cavernous nerve injury rat model
Yi-No Wu1, Chun-Hou Liao 2,3, Chien-Chih Wu4,5, Han-Sun Chiang1,2,5
Graduate Institute of Basic Medicine, Fu Jen Catholic University, New Taipei City, Taiwan1; Division of Urology, Department of Surgery, Cardinal Tien Hospital2; College of Medicine, Fu Jen Catholic University3 ; School of Medicine, Taipei Medical University, Taipei, Taiwan4; Department of Urology, Taipei Medical University Hospital, Taipei, Taiwan5
Purpose:
The purpose of the present study was to determine the platelet rich plasma (PRP) action target that mediates its effect on erectile function (EF) recovery in the bilateral cavernous nerve crush (BCNC) injury rat model.
Materials and Methods:
52 rats were randomly divided into two equal groups: intracavernosal injection (IC) of saline after BCNC (group 1) and IC injection of PRP after BCNC (group 2). Five animals in each group were euthanized at 3 and 7 day (d) post-injection, and the tissues were harvested to conduct transmission electron microscopy (TEM) and histological assays in the penis, cavernous nerve (CN), and major pelvic ganglion (MPG). Eight animals in each group were used to determine the recovery of EF at 14 and 28 d post-injury, after which the penis, CN, and MPG tissues were harvested to conduct TEM and histological assays.
Results:
Intracavernosal injections of PRP increased all erectile function parameters at 28 d (p<0.05) but not 14 d (p=0.55) post-injury. PRP injections simultaneously prevented the loss of neural nitric oxide synthase (nNOS)-positive neurons (p<0.05) and nerve fibers (p<0.05) in the MPG and CN compared with group 1, respectively, which accelerated the regeneration of myelinated axons of the CN, reduced apoptosis, and enhanced the proliferation of the corporal smooth muscle cells at an earlier stage.
Conclusions:
The results of this study suggest that the application of PRP for erectile dysfunction caused by BCNC-related axonotmesis was beneficial to restore EF via both neuroprotective and tissue-protective effects.