Human glutathione S-transferase omega rs4925 polymorphism與膀胱癌之關聯
黃鐘銘、許兆畬、童敏哲、吳佳璋1、王淵宏1
童綜合醫院 外科部 泌尿外科；1雙和醫院 泌尿外科
Association between human glutathione s-transferase omega rs4925 polymorphism and bladder cancer
Zhon-Min Huang, Jow-Yu Sheu, Min-Che Tung, Chia-Chang Wu1, Yuan-Hung Wang1
Divisions of Urology, Department of Surgery, Tungs’ Taichung Metro Harbor Hospital, Taichung, Taiwan
1Divisions of Urology, Shuang Ho Hospital, Taipei Medical University New Taipei City Taiwan
Purpose:
Glutathione S-transferases (GSTs) play an important role in the detoxification of polycyclic aromatic hydrocarbons and aromatic amines, the toxic substances contained in cigarettes. GST Omega 1 (GSTO1) not only utilizes glutathione in conjugation reaction but also contributes to the biotransformation of several xenobiotics. A single nucleotide polymorphism (Ala- 140Asp) of GSTO1 gene causing variations in enzyme activity may influence individual susceptibility to bladder cancer (BC). It is hypothesized that genetic polymorphism of GSTO1 gene has an effect on BC risk in particular by interacting with cigarette smoking.
Materials and Methods:
A total of histopathologically confirmed 300 BC patients and 300 cancer-free controls were recruited from February 2002 to February 2009. Genotyping of the GSTO1 Ala140Asp polymorphism was deter- mined using a polymerase chain reaction-restricted fragment length polymorphism (PCR-RFLP) method. The odds ratio (OR) and 95% confidence interval (CI) were calculated as a measure of the combined effect of cigarette smoking and the GSTO1 Ala140Asp polymorphism on BC risk.
Results:
We found that study subjects with the GSTO1 Ala/Ala genotype have a significantly increased BC risk (OR = 1.5; 95% CI = 1.1 - 2.7). A statistically significant increased BC risk was also found in ever smokers with the GSTO1 Ala/Ala genotype (OR = 4.9; 95%CI = 2.8 - 9.7).
Conclusions:
This study provides an epidemiologic evidence of a significantly increased BC risk among ever smokers with the GSTO1 Ala/Ala genotype.