TGR5可能在高脂肪飼料老鼠引發膀胱功能變化
陳奕宏、鄭瑞棠1,2、唐一清
國立成功大學醫學院附設醫院泌尿科；1奇美醫學中心醫學研究部；2長榮大學醫學研究所
TGR5 COULD MEDIATE BLADDER FUNCTION CHANGE IN RATS FED WITH HIGH-FAT DIET
I-Hung Chen , Juei-Tang Cheng1,2, Yat-Ching Tong
Department of Urology, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan City, Taiwan; Department of Medical Research, Chi-Mei Medical Center, Tainan City, Taiwan1; Institute of Medical Sciences, Chang Jung Christian University, Tainan City, Taiwan2
Purpose: To investigate the role of TGR5 in bladder function in rats fed with high-fat diet.
Materials and Methods: Male Sprague-Dawley rats were fed with high-fat diet for eight weeks to induce metabolic syndrome. Another group was rats fed with normal chow. Filling cystometry was performed and voiding function was observed after rats were anesthetized. During cystometry testing, betulinic acid, an agonist of TGR5, and triamterene, an antagosit of TGR5, were mixed into infusion saline in different concentration to explore the effect of TGR5 on bladder storage and voiding. Then bladder was harvested and western blot was applied for TGR5 receptors expression.
Results: Metabolic syndrome was successfully induced in rats fed with high-fat diet. Increased bladder capacity, increased voided volume, reduced voiding efficacy, diminished bladder contractile pressure and decreased bladder contraction episodes in thirty minutes were found in rats fed with high-fat diet. The same results was found in normal rats which received cystometry with infusion containing betulinic acid. Besides, the all bladder function change mentioned above was reversed by adding triamterene into infusion during cystometry testing. Up-regulation of TGR5 receptor protein was found in rats fed with high-fat diet.
Conclusion: TGR5 might play a role in alteration of bladder function in rats fed with high-fat diet.